单水平生物标志物检测在癌症诊断中存在准确性不足的局限性。因此，开发用于高通量 ctDNA 测定的高灵敏度、多通道生物传感器对于提高临床肿瘤早期诊断的准确性至关重要。为了实现卵巢癌早期诊断多达10个靶标的高效检测，基于多模块催化发夹组装介导的信号放大，构建了基于DNA纳米开关的多通道（DNA-NSMC）生物传感器(CHA) 和立足点介导的 DNA 链置换 (TDSD) 反应。仅使用两种不同的荧光信号作为输出，结合基于DNA纳米开关的反应平台的模块化分割策略；首次成功实现了多达10个目标的多通道检测。实验结果表明，所提出的生物传感器是一种有前途的工具，可同时检测多种生物标志物，用于卵巢癌的早期诊断，为卵巢癌和其他癌症的早期筛查、诊断和治疗提供新策略。© 2024作者获得 Springer-Verlag GmbH Austria（Springer Nature 旗下公司）的独家许可。

Single-level biomarker detection has the limitation of insufficient accuracy in cancer diagnosis. Therefore, the strategy of developing highly sensitive, multi-channel biosensors for high-throughput ctDNA determination is critical to improve the accuracy of early diagnosis of clinical tumors. Herein, in order to achieve efficient detection of up to ten targets for early diagnosis of ovarian cancer, a DNA-nanoswitch-based multi-channel (DNA-NSMC) biosensor was built based on the multi-module catalytic hairpin assembly-mediated signal amplification (CHA) and toehold-mediated DNA strand displacement (TDSD) reaction. Only two different fluorescence signals were used as outputs, combined with modular segmentation strategy of DNA-nanoswitch-based reaction platform; the multi-channel detection of up to ten targets was successfully achieved for the first time. The experimental results suggest that the proposed biosensor is a promising tool for simultaneously detecting multiple biomarkers for the early diagnosis of ovarian cancer, offering new strategies for the early screening, diagnosis, and treatment not only for ovarian cancer but also for other cancers.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.