姜黄素（CUR）对肿瘤生长具有潜在的抑制作用；然而，其疏水性和不稳定性限制了其临床应用。在本研究中，我们开发了 CUR 纳米颗粒（CUR-NP）并评估了其生化特性。分别使用划痕实验和Transwell实验评估细胞摄取和增殖。 Western blotting检测SKOV3细胞中NF-κB/PRL-3信号通路、炎症反应、细胞增殖和细胞迁移相关蛋白的表达水平。我们的研究结果表明，空白载体对细胞没有细胞毒性，使我们能够忽略载体本身引起的任何影响。 CUR-NPs 对细胞增殖表现出浓度和时间依赖性的抑制作用，超过了单独使用 CUR 的效果。增加 CUR-NP 的浓度会导致细胞划痕愈合能力降低和室迁移能力降低。与对照组相比，NF-κB/PRL-3信号通路、炎症反应（TNF-α和IL-6）、细胞增殖（cyclin E1和cyclin A1）以及细胞迁移相关蛋白的表达水平（ N-钙粘蛋白和波形蛋白）在脂多糖（LPS）刺激和 NF-κB p65 过表达组中显着升高。相反，E-钙粘蛋白表达在这些条件下显着降低。然而，用高浓度的 CUR-NP 处理可以有效逆转这些变化。这些结果凸显了 CUR-NP 抑制人卵巢癌细胞增殖和迁移的显着能力，同时通过调节 NF-κB/PRL-3 信号通路抑制炎症反应。版权所有 © 2024 作者。由 Elsevier B.V. 出版。保留所有权利。

Curcumin (CUR) exhibits potential inhibitory effects on tumor growth; however, its hydrophobicity and instability limit its clinical applications. In the present study, we developed CUR nanoparticles (CUR-NPs) and evaluated their biochemical characteristics. Cell uptake and proliferation were assessed using scratch and Transwell assays, respectively. Western blotting was performed to investigate the expression levels of proteins related to the NF-κB/PRL-3 signaling pathway, inflammatory response, cell proliferation, and cell migration in SKOV3 cells. Our findings showed that the blank vector was not cytotoxic to cells, allowing us to disregard any effects caused by the vector itself. CUR-NPs exhibited concentration- and time-dependent inhibitory effects on cell proliferation, surpassing those of CUR alone. Increasing the concentration of CUR-NPs resulted in a reduced cell scratch-healing ability and lower chamber migration capacity. Compared to the control group, expression levels of proteins associated with NF-κB/PRL-3 signaling pathway, inflammatory response (TNF-α and IL-6), cell proliferation (cyclin E1 and cyclin A1), as well as cell migration (N-cadherin and vimentin) were significantly elevated in the lipopolysaccharide (LPS) stimulation and NF-κB p65 overexpression groups. Conversely, E-cadherin expression was significantly decreased under these conditions. However, treatment with high concentrations of CUR-NPs effectively reversed these changes. These results highlight the significant ability of CUR-NPs to inhibit human ovarian cancer cell proliferation and migration, while suppressing inflammatory responses through the regulation of the NF-κB/PRL-3 signaling pathway.Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.