光动力疗法（PDT）已成为不可切除胆管癌（CCA）的一种有前景的治疗方法。大量实验表明PDT可以显着增强抗肿瘤免疫力。然而，PDT对不可切除CCA外周免疫系统的影响仍不清楚。在一项比较PDT支架治疗和单独支架治疗不可切除CCA围手术期和长期结果的临床试验中，我们测试了淋巴细胞（CD4 T细胞）的水平、CD8 T 细胞、NK 细胞、B 细胞和 Treg 细胞）和免疫相关细胞因子（IL-4、IL-6、IL-10、TNF-α、TGF-β、穿孔素、GM-CSF 和 IFN-γ）检测PDT支架治疗或单纯支架治疗前后外周血的变化，分析PDT对不可切除CCA外周免疫系统的影响。支架组和单独支架组。 PDT治疗后CD8+T细胞比例显着增加，而其他种类淋巴细胞无显着差异。 PDT治疗后IL-6水平升高，穿孔素和TGF-β水平降低，而其他免疫相关细胞因子没有明显变化。PDT改变了外周血中免疫细胞和免疫相关细胞因子的水平不可切除的 CCA 患者，可能与 PDT 在不可切除的 CCA 治疗中的疗效相关。未来的研究可以更深入地研究这一方面，探索如何更有效地利用 PDT 来治疗不可切除的 CCA。版权所有 © 2024。由 Elsevier B.V. 出版。

Photodynamic therapy (PDT) has been emerging as a promising treatment for unresectable cholangiocarcinoma (CCA). A number of experiments have demonstrated that PDT could enhance antitumor immunity significantly. However, the impact of PDT on peripheral immune system for unresectable CCA remains unclear.In a clinical trial comparing the perioperative and long-term outcomes of PDT+stent treatment and stent alone treatment for unresectable CCA, we tested the levels of lymphocytes (CD4+ T cells, CD8+ T cells, NK cells, B cells and Treg cells) and immune-related cytokines (IL-4, IL-6, IL-10, TNF-α, TGF-β, perforin, GM-CSF and IFN-γ) in peripheral blood before and after PDT+stent treatment or stent alone treatment and analyzed the influence of PDT on peripheral immune system for unresectable CCA.Before treatment, the levels of all the immune cells and immune-related cytokines did not show significant differences between the PDT+stent group and stent alone group. The ratio of CD8+ T cells increased significantly after PDT treatment, but other kinds of lymphocytes did not show significant difference. Increased level of IL-6 and decreased level of perforin and TGF-β after PDT treatment were demonstrated, whereas no significant changes were found for other immune-related cytokines.PDT altered the levels of immune cells and immune-related cytokines in the peripheral blood of unresectable CCA patients, potentially correlating with the therapeutic efficacy of PDT in unresectable CCA treatment. Future studies could delve deeper into this aspect to explore how PDT can be more effectively utilized in the management of unresectable CCA.Copyright © 2024. Published by Elsevier B.V.