Clinacanthus nutans (C. nutans) 是一种产于热带亚洲的植物，具有已证实的生物活性。 C. nutans 粗多糖 (CNP) 的优化提取方法在超声波辅助机械方法 (UL_CNP) 存在下使用水。然而，使用 UL_CNP 来合成和优化银纳米粒子 (AgNP)，特别是其抗癌和光催化特性，仍有待探索。因此，本研究旨在采用绿色方法，使用 UL_CNP 和硝酸银生产小尺寸的银纳米粒子 (UL_AgNP)，并评估其潜在的毒性、抗癌和光催化活性。采用Box-Behnken设计方法优化合成条件。合成的UL_AgNP在458nm处显示出表面等离子体共振峰。优化的合成条件产生了球形的UL_AgNP，尺寸为5.21±1.92nm，zeta电位为-26.33±0.93mV。 X射线衍射分析在(111)、(200)、(220)和(311)处显示出强烈的布拉格反射峰，具有面心立方结构的AgNP。衰减全反射傅里叶变换红外光谱和能量色散X射线光谱进一步证实了合成的UL_AgNP中银的存在。 UL_AgNP 丰年虾致死率测试显示，24 小时后致死浓度 50 值<7.8μg/mL。 UL_AgNP 通过诱导 S 期细胞周期停滞、凋亡作用和细胞迁移减少，对 MCF-7 细胞表现出抗增殖活性，半数抑制浓度值为 4.96±0.31μg/mL。此外，UL_AgNP 证明了其对亚甲基蓝染料的高效光催化活性（50μg/mL 浓度下 10 分钟后为 50.22%±0.06%）。因此，UL_AgNP 对 MCF-7 细胞表现出有希望的抗增殖活性，突出了它们作为治疗剂的潜力。需要进一步研究以阐明其作用的精确机制。版权所有 © 2024。由 Elsevier B.V. 出版。

Clinacanthus nutans (C. nutans) is a plant in tropical Asia with proven biological activities. The optimized extraction method of C. nutans crude polysaccharide (CNP) uses water in the presence of an ultrasound-assisted mechanical method (UL_CNP). However, the use of UL_CNP for the synthesis and optimization of silver nanoparticles (AgNP), particularly their anticancer and photocatalytic properties, remains unexplored. Hence, this research aimed to employ a green method using UL_CNP and silver nitrate to produce AgNP (UL_AgNP) with a small size and assess its potential toxicity, anticancer, and photocatalytic activities. The synthesis condition was optimized using the Box-Behnken design method. The synthesized UL_AgNP showed the surface plasmon resonance peak at 458 nm. The optimized synthesis condition produced spherically shaped UL_AgNP with a size of 5.21 ± 1.92 nm and a zeta potential of -26.33 ± 0.93 mV. An X-ray diffraction analysis exhibited intense Bragg's reflection peaks at (111), (200), (220), and (311), having a face-centered cubic structure of AgNP. Attenuated total reflectance-Fourier-transform infrared spectroscopy and energy-dispersive X-ray spectroscopy further confirmed the presence of silver in the synthesized UL_AgNP. The brine shrimp lethality test of UL_AgNP reported a lethal concentration 50 value of <7.8 μg/mL after 24 h. The UL_AgNP exhibited antiproliferative activity against MCF-7 cells with a half-maximal inhibitory concentration value of 4.96 ± 0.31 μg/mL by inducing S-phase cell cycle arrest, apoptotic effect, and reduction of cell migration. Furthermore, UL_AgNP proved its efficient photocatalytic activity against methylene blue dye (50.22 % ± 0.06 %, after 10 min at a concentration of 50 μg/mL). Therefore, the UL_AgNP exhibited promising antiproliferative activity against MCF-7 cells, highlighting their potential as a therapeutic agent. Further investigations are needed to elucidate the precise mechanism of their action.Copyright © 2024. Published by Elsevier B.V.