诱导多能干细胞 (iPSC) 如今是广泛应用的常见起点，包括 3D 疾病模型（即类器官）和未来再生医学。体内平衡、细胞分化、发育和繁殖等生理过程受到激素通过与靶细胞的跨膜或核受体结合而严格调节。考虑到它们的多效性作用，同时考虑它们在基于 iPSC 的疾病模型中的表达，将更好地概括导致 3D 类器官发育和疾病研究的分子事件。在这里，我们报道了四种不同 iPSC 中雌激素受体 (ERα) 和孕激素受体 (PR) 的表达模式，这些 iPSC 是通过四种不同方法从 CD34  祖细胞和皮肤成纤维细胞获得的。与 MCF7 阳性对照相比，iPSC 和成纤维细胞中 ERα 和 PR mRNA 的表达显着下调。免疫荧光 (IF) 染色仅检测到所有不同 iPSC 细胞系中 PR 蛋白的表达，而未检测到 ERα。通过流式细胞术分析，我们观察到iPSCs细胞总数的65%仅表达PR，与HSPCs和成纤维细胞相比增加了100%倍，而ERα不表达。我们的结果首次共同证明，体细胞重编程为 iPSC 会导致 PR 受体的表达。© 2024。作者。

Induced Pluripotent Stem Cells (iPSCs) are nowadays a common starting point for wide-ranging applications including 3D disease modeling (i.e. organoids) and in future regenerative medicine. Physiological processes like homeostasis, cell differentiation, development and reproduction are tightly regulated by hormones through binding to their transmembrane or nuclear receptors of target cells. Considering their pleiotropic effect, take into account also their expression in an iPSCs-based disease modeling would better recapitulate the molecular events leading to 3D organoid development and disease study. Here we reported the expression pattern of estrogen receptor (ERα) and progesterone receptor (PR) in four different iPSCs, obtained from CD34 + progenitor cells and skin fibroblasts with four different methods. Expression of ERα and PR mRNA were significantly downregulated in iPSCs as well as fibroblasts compared to MCF7 positive control. Immunofluorescence (IF) staining detected only the expression of PR protein in all the different iPSCs cell lines, while ERα was not detectable. By flow cytometry analysis we observed that the ~ 65% of the total population of iPSCs cells expressed only PR, with 100% fold increase compared to HSPCs and fibroblasts, while ERα was not expressed. Our results collectively demonstrated for the first time that the reprogramming of somatic cells into iPSCs leads to the expression of PR receptor.© 2024. The Author(s).