癌症干细胞（CSC）在肿瘤对化疗的抵抗以及胃癌的快速增殖和转移中发挥着关键作用。我们的目的是探讨胃癌上皮细胞中的CSCs相关基因。从公共数据库下载胃癌的mRNA表达谱和单细胞测序数据。我们鉴定WDR72为胃癌上皮细胞中的CSCs相关基因。 WDR72在胃癌组织中高表达，WDR72高表达与患者预后不良相关。 WDR72表达与CD8 T细胞和活化记忆CD4 T细胞的浸润呈显着负相关。 WDR72 高表达的胃癌患者中 PD-L1 表达显着降低。 WDR72 与多种小分子药物的 IC50 相关。我们在胃癌上皮细胞中发现了一种新的 CSC 相关基因 WDR72，该基因在高干性评分的患者中高表达。© 2024 作者。

Cancer stem cells (CSCs) are pivotal in tumor resistance to chemotherapy and gastric cancer's rapid proliferation and metastasis. We aimed to explore the CSCs-related genes in gastric cancer epithelial cells.The mRNA expression profile and single-cell sequencing data of gastric cancer were downloaded from the public database.We identified WDR72 as a CSCs-related gene in gastric cancer epithelial cells. WDR72 was highly expressed in gastric cancer tissues, and high expression of WDR72 was associated with inferior prognosis of patients. WDR72 expression had a significant negative correlation with the infiltration of CD8 + T cells and activated memory CD4 + T cells. PD-L1 expression was significantly reduced in gastric cancer patients with high WDR72 expression. WDR72 was correlated with IC50 of multiple small-molecule drugs.We identified a novel CSCs-related gene in gastric cancer epithelial cells, WDR72, which was highly expressed in patients with high stemness scores.© 2024 The Authors.