使用 GC-MS 和 FTIR 研究埃塞俄比亚药用植物的生物活性植物化学成分。
Investigation of bioactive phytochemical compounds of the Ethiopian medicinal plant using GC-MS and FTIR.
发表日期:2024 Aug 15
作者:
Yassin Adem Endris, Kedir Yesuf Abdu, Solomon Getachew Abate
来源:
ANTIOXIDANTS & REDOX SIGNALING
摘要:
药用植物高芳香族原料可作为手术治疗的替代药物用于治疗疣,因为它们可以从体内永久去除。因此,本研究旨在从 Calotropis procera 叶子中提取植物材料,描述植物化学筛选,分析抗菌活性,确定 FTIR 中的官能团,并鉴定 GC-MS 中的化合物。测得牛角瓜粗提物的 PH、比重和粘度分别为 4.5、0.79 和 0.49。分析粗提物的溶解度;乙醇可以溶解,而水则不能。牛角瓜提取物的植物化学筛选试验中还含有黄酮类、生物碱、酚类、单宁酸和皂苷,而粗提取物中不存在三萜类、萜类化合物和类固醇。类黄酮、生物碱、酚类、单宁和皂苷是治疗性植物材料中发现的主要植物化学成分。通过 FT-IR 分析牛角瓜粗提物的官能团,含有羟基、烷烃、羰基、醛、酮、酚、酯、醇和亚甲基。通过GC-MS对牛角瓜原料进行化学成分分析,发现主要化合物有22种。在 22 种化合物中,有 5 种化合物是用于医疗用途的活性成分。牛角瓜植物提取物中发现的生物活性化合物是新植二烯、六氢法尼基、羊毛甾醇、2,4-二甲基苯并[H]喹诺酮和角鲨烯。这些生物活性化合物具有抗菌、镇痛、解热、抗炎、抗菌、抗氧化、抗病毒和抗癌特性。在体外抗菌活性测试中,粗提物对革兰氏阳性菌的抑制作用多于对革兰氏阴性菌的抑制作用。这个集体原因就是传统治疗师使用这种 Calotropis procera 植物来治疗疣的原因。© 2024 作者。
Medicinal plants Highly aromatic crude materials are utilized for treating warts as an alternative medicine to surgical treatment because they can be permanently removed from the body. Thus, this investigation aimed to extract plant material from Calotropis procera leaves, describe the phytochemical screening, analyze anti-microbial activities, determine the functional groups in FTIR, and identify the chemical compounds in GC-MS. The PH, specific gravity, and viscosity of the crude extracts of Calotropis procera were determined at 4.5, 0.79, and 0.49, respectively. Analyze the solubility of crude extracts; ethanol can dissolve while water does not. Flavonoids, alkaloids, phenols, tannins, and saponins were also present in the phytochemical screening tests of the Calotropis procera extracts, triterpenoids, terpenoids, and steroids were not present in the crude extract. Flavonoids, alkaloids, phenols, tannins, and saponins are the primary phytochemical components found in therapeutic plant material. The Calotropis procera crude extracts analyzed for functional groups by FT-IR contained a hydroxyl group, alkane, carbonyl, aldehyde, ketone, phenols, ester, alcohol, and methylene. The chemical compounds analyzed by GC-MS of Calotropis procera crude material were found to have 22 main compounds. Of 22 compounds, 5 compounds are active ingredients for the applications of medical purposes. The bioactive compounds found in the Calotropis procera plant extract are neophytadiene, hexahydrofarnesyl, lanosterol, 2,4-dimethylbenzo [H]quinolone, and squalene. Those bioactive compounds have anti-bacterial, analgesic, antipyretic, anti-inflammatory, antimicrobial, antioxidant, antiviral, and anti-cancer properties. In an in vitro antimicrobial activity test, the crude extract effectively inhibited more gram-positive bacteria than gram-negative bacteria. This collective reason is why the traditional therapist uses this Calotropis procera plant for the treatment of warts.© 2024 The Authors.