在胃肠道间质瘤（GIST）领域，了解分子格局和预后因素对于有效治疗至关重要。脱碘酶 3 基因 (DIO3) 以其在甲状腺激素调节和细胞增殖中的作用而闻名，已成为 GIST 发病机制的潜在参与者。我们的研究调查了 GIST 样本中的 DIO3 表达及其与肿瘤特征的相关性，旨在加强预后分层和个性化治疗策略。我们采用回顾性设计，分析了诊断为 GIST 患者的数据和福尔马林固定石蜡包埋 (FFPE) 样本。该研究队列由 33 名患者组成，主要是女性，中位年龄为 66 岁。仔细记录肿瘤特征，包括位置、大小、有丝分裂计数、风险分类和免疫组织化学标记物。使用 FFPE 样本进行 DIO3 基因表达分析，重点是相对定量以及与免疫组化标记物和预后风险的关联。在 69.70% 的肿瘤中观察到 DIO3 过表达，而在 30.30% 的病例中观察到表达不足。关联分析揭示了有趣的见解。在 DIO3 表达和 DOG1 频率之间发现了显着的关联，表明这些标记物在 GIST 病理学中存在潜在的相互作用。此外，在极低/低风险预后患者中，DIO3 表达增加显着较高，暗示 DIO3 水平与肿瘤进展预后之间可能存在联系。 本研究中发现的 DIO3 表达与 GIST 特征之间错综复杂的相互作用强调了分子标记在完善 GIST 患者预后评估和治疗策略方面的潜力。版权所有 © 2024，Kolcsár 等人。

In the realm of gastrointestinal stromal tumors (GIST), understanding the molecular landscape and prognostic factors is crucial for effective management. The deiodinase 3 gene (DIO3), known for its role in thyroid hormone regulation and cell proliferation, has emerged as a potential player in GIST pathogenesis. Our study investigated DIO3 expression in GIST samples and its correlation with tumor characteristics, aiming to enhance prognostic stratification and personalized treatment strategies.Using a retrospective design, we analyzed data and formalin-fixed paraffin-embedded (FFPE) samples of patients diagnosed with GIST. The study cohort comprised 33 patients, predominantly female, with a median age of 66 years. The tumor characteristics were meticulously documented, including location, size, mitotic count, risk classification, and immunohistochemical markers. Gene expression analysis of DIO3 was conducted using FFPE samples, with a focus on relative quantification and association with immunohistochemical markers and prognostic risk.DIO3 overexpression was observed in 69.70% of tumors, while underexpression was noted in 30.30% of cases. Association analyses revealed intriguing insights. A notable association was identified between DIO3 expression and the frequency of DOG1, suggesting a potential interplay between these markers in GIST pathobiology. Furthermore, increased DIO3 expression was significantly higher in very low/low-risk prognostic patients, hinting at a possible link between DIO3 levels and tumor progression prognosis. The intricate interplay between DIO3 expression and GIST characteristics uncovered in this study underscores the potential of molecular markers in refining prognostic assessments and therapeutic strategies for GIST patients.Copyright © 2024, Kolcsár et al.