单细胞技术被认为是在单细胞分辨率下研究肿瘤生物系统的先进而强大的工具，并且在药物发现和开发的多个阶段发挥着越来越重要的作用。具体来说，单细胞技术可以促进药物靶点的发现，有助于单细胞水平的高通量筛选，并有助于抗肿瘤药物的药代动力学研究。新兴的单细胞分析技术已经发展到进一步整合多维单细胞分子特征，扩大单细胞数据规模，分析单细胞基因的表型影响，并提供全长覆盖的单细胞测序。在这篇综述中，我们系统地总结了单细胞技术在肿瘤治疗药物发现的各个领域的应用，包括靶点识别、高通量药物筛选和药代动力学评价，并重点介绍了新兴的单细胞技术在提供深入理解方面的作用。肿瘤生物学。基于单细胞技术的药物发现有望进一步优化治疗策略并改善肿瘤患者的临床结果。© 2024 作者。

Single-cell technologies have been known as advanced and powerful tools to study tumor biological systems at the single-cell resolution and are playing increasingly critical roles in multiple stages of drug discovery and development. Specifically, single-cell technologies can promote the discovery of drug targets, help high-throughput screening at single-cell level, and contribute to pharmacokinetic studies of anti-tumor drugs. Emerging single-cell analysis technologies have been developed to further integrating multidimensional single-cell molecular features, expanding the scale of single-cell data, profiling phenotypic impact of genes in single cell, and providing full-length coverage single-cell sequencing. In this review, we systematically summarized the applications of single-cell technologies in various sections of drug discovery for tumor treatment, including target identification, high-throughput drug screening, and pharmacokinetic evaluation and highlighted emerging single-cell technologies in providing in-depth understanding of tumor biology. Single-cell-technology-based drug discovery is expected to further optimize therapeutic strategies and improve clinical outcomes of tumor patients.© 2024 The Authors.