我们的目标是研究环糊精在生产肉桂水提取物中的用途，该提取物具有高含量的生物活性成分，并通过基于细胞的测定进行验证。由于其安全性和成本效益，天然化合物在癌症治疗中引起了人们的关注。经常面临与药物毒性和耐药性相关的挑战。肉桂（Cinnamomum verum；也称为锡兰肉桂）是一种常用香料，在民间医学中具有治疗各种疾病的历史。然而，其活性成分溶解度、稳定性和生物利用度较差，限制了其使用和益处。我们制备了γ环糊精（γCD）辅助的肉桂水提取物（CD-CIN），并将其活性与DMSO提取物（DM-CIN）进行了比较。在正常和应激（氧化、金属和缺氧）条件下将细胞暴露于 CD-CIN 和 DM-CIN 提取物，然后使用各种分子测定法分析应激和癌表型。我们发现 CD-CIN 具有相当大的抗癌活性涉及肿瘤抑制蛋白的激活和 DNA 损伤反应。分子标记分析支持，低、无毒浓度的 DM-CIN 和 CD-CIN 对细胞的迁移和侵袭能力产生了相当的抑制。此外，与对照相比，DM-CIN 和 CD-CIN 处理的细胞均记录了针对氧化、金属和缺氧应激的保护以及分化诱导。我们将 CD-CIN 报告为一种新的经济且易于使用的细胞。肉桂来源的资源具有相当大的抗癌和抗应激活性，因此值得进一步的化学、体外和体内研究。版权所有© Bentham Science Publishers；如有任何疑问，请发送电子邮件至 epub@benthamscience.net。

Our goal was to investigate the use of Cyclodextrin in creating an aqueous extract of Cinnamon with a high content of its bioactive ingredients, validated by cell-based assays.Due to their safety and cost-effectiveness, natural compounds have garnered attention for cancer therapy, which often faces challenges related to drug toxicity and resistance. Cinnamon (Cinnamomum verum; also known as Ceylon Cinnamon) is a commonly used spice with a history in folk medicine for treating various ailments. However, its active ingredients suffer from poor solubility, stability, and bioavailability, which limits its use and benefits.We prepared γCyclodextrin (γCD)-assisted aqueous extract of Cinnamon (CD-CIN) and compared its activity with the DMSO extract (DM-CIN).The cells were exposed to CD-CIN and DM-CIN extracts under normal and stressed (oxidative, metal, and hypoxic) conditions and then analyzed for stress and cancerous phenotypes using various molecular assays.We found that CD-CIN possesses considerable anticancer activity that involves the activation of tumor suppressor proteins and DNA damage response. Low, non-toxic concentrations of DM-CIN and CD-CIN caused comparable inhibition of migration and invasion capability of cells, supported by molecular marker analyses. Furthermore, protection against oxidative, metal, and hypoxia stress, as well as induction of differentiation, was recorded in both DM-CIN and CD-CIN treated cells, as compared to the control.We report CD-CIN as a new economic and easy Cinnamon-derived resource that possesses considerable anticancer and antistress activities and hence warrants further chemical, in vitro, and in vivo studies.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.