细胞外囊泡（EV）因其在诊断和治疗炎症、自身免疫性疾病和癌症方面的潜力而引起了广泛的兴趣。 EV 是脂质囊泡，由称为外泌体的内体来源的囊泡、膜脱落的微泡以及程序性细胞死亡膜起泡的凋亡小体组成，其携带来自其起源细胞的复杂货物组，包括蛋白质、脂质、mRNA 和 DNA。 EV 富含整合素蛋白，可促进内在细胞通讯以传递其货物内容，也可用作研究各自细胞状况的生物标志物。在此背景下，我们假设当这些 EV 与合成脂质体杂交时，将有助于在复杂的生物环境中导航混合结构以找到其目标。为了实现这一目标，我们将合成脂质体与源自小鼠乳腺癌（4T1肿瘤）细胞的EV（本文称为LEV）杂交，并掺入罗丹明-B/近红外荧光染料，以研究其细胞靶向和肿瘤递送的潜力。使用膜挤出，我们成功地杂交了这两种实体，从而形成了 LEV，并表征了它们在一段时间内的胶体特性和稳定性。虽然 EV 是广泛分散的纳米和微米大小的囊泡，但 LEV 被设计为单分散的，平均流体动力学尺寸为 140 ± 5。使用免疫印迹和 ELISA，我们监测和量化了 EV 特异性蛋白 CD63 和其他特征蛋白（例如 CD9）和 CD81，它们被用作确保 EV 和 LEV 的可重复性的手柄。这些 LEV 进一步受到带有原位 4T1 乳腺肿瘤的小鼠的攻击，在活体动物成像中与对照聚乙二醇化脂质体相比，发现肿瘤和肝脏、脾脏和肺等器官中的 LEV 摄取量最大。同样，这些构建体能够如离体成像中观察到的那样发现肺转移。我们预计这项研究可以为在目标识别方面表现出色的药物输送解决方案开辟途径。

Extracellular vesicles (EVs) have gained widespread interest due to their potential in the diagnosis and treatment of inflammation, autoimmune diseases, and cancers. EVs are lipidic vesicles comprising vesicles of endosomal origin called exosomes, microvesicles from membrane shedding, and apoptotic bodies from programmed cell death membrane blebbing that carry complex sets of cargo from their cells of origin, including proteins, lipids, mRNA, and DNA. EVs are rich in integrin proteins that facilitate intrinsic cellular communication to deliver their cargo contents and can also be used as biomarkers to study respective cellular conditions. Within this background, we hypothesized that when these EVs are hybridized with synthetic liposomes, it would help navigate the hybrid construct in the complex biological environment to find its target. Toward this endeavor, we have hybridized a synthetic liposome with EVs (herein called LEVs) derived from mouse breast cancer (4T1 tumors) cells and incorporated a rhodamine-B/near-infrared fluorescent dye to investigate their potential for cellular targeting and tumor delivery. Using membrane extrusion, we have successfully hybridized both entities resulting in the formation of LEVs and characterized their colloidal properties and stability over a period. While EVs are broadly dispersed nano- and micron-sized vesicles, LEVs are engineered as monodispersed with an average hydrodynamic size of 140 ± 5. Using immunoblotting and ELISA, we monitored and quantified the EV-specific protein CD63 and other characteristic proteins such as CD9 and CD81, which were taken as a handle to ensure the reproducibility of EVs and thus LEVs. These LEVs were further challenged with mice bearing orthotopic 4T1 breast tumors and the LEV uptake was found to be maximum in tumors and organs like the liver, spleen, and lungs when compared to control PEGylated liposomes in live animal imaging. Likewise, the constructs were capable of finding lung metastasis as observed in ex vivo imaging. We anticipate that this study can open avenues for drug delivery solutions that are superior in target recognition.