通过将近红外光与 MoS2/CuO/Au 纳米复合材料相结合来构建比色免疫分析，开发了一种新颖的信号放大策略。首先，通过沉淀和光还原方法合成了MoS2/CuO/Au纳米复合材料，并通过扫描电子显微镜（SEM）和X射线粉末衍射（XRD）对其进行了表征。 MoS2/CuO/Au纳米复合材料具有氧化酶样活性，可以氧化TMB形成蓝色产物（TMBox）。此外，TMB 的催化氧化在近红外 (NIR) 激光辐射下加速。使用MoS2/CuO/Au纳米复合材料构建夹心式比色免疫分析法。在近红外光增强下，可灵敏地检测到0.1至40 ng/mL范围内的癌胚抗原（CEA），检出限为0.03 ng/mL。此外，该免疫传感器具有优异的选择性和抗干扰性、良好的重复性和稳定性。© 2024。作者获得 Springer-Verlag GmbH Austria（Springer Nature 旗下公司）的独家许可。

A novel signal amplification strategy was developed by combining near-infrared light with MoS2/CuO/Au nanocomposite for building a colorimetric immunoassay. First, MoS2/CuO/Au nanocomposite was synthesized by precipitation and photoreduction methods and characterized by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD). MoS2/CuO/Au nanocomposite has oxidase-like activity and can oxidize TMB to form a blue product (TMBox). Further, the catalytic oxidation of TMB was accelerated under near-infrared (NIR) laser radiation. The sandwich-type colorimetric immunoassay was constructed using MoS2/CuO/Au nanocomposite. Under the enhancement of near-infrared light, carcinoembryonic antigen (CEA) was sensitively detected in the range 0.1 to 40 ng/mL with the limit of detection of 0.03 ng/mL. Moreover, the immunosensor has excellent selectivity and anti-interference, good repeatability, and stability.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.