用于量化 3D 患者衍生卵巢癌模型中药物敏感性的方案。
Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models.
发表日期：2024 Aug 21
作者：
Kathrin B Labrosse, Flavio C Lombardo, Natalie Rimmer, Mónica Núñez López, André Fedier, Viola Heinzelmann-Schwarz, Ricardo Coelho, Francis Jacob
来源：
BIOMEDICINE & PHARMACOTHERAPY
摘要：
三维 (3D) 离体培养物可用于研究癌症进展和耐药机制。在这里，我们提出了一种通过定量凋亡肿瘤细胞来测量无支架 3D 培养系统中的靶向药物敏感性的方案。我们提供样品处理、免疫荧光染色、半高通量共焦成像和 3D 培养物基于图像的定量的详细步骤。该协议用途广泛，原则上可应用于任何源自患者的材料。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。
Three-dimensional (3D) ex vivo cultures allow the study of cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring on-target drug sensitivity in a scaffold-free 3D culture system through quantification of apoptotic tumor cells. We provide detailed steps for sample processing, immunofluorescence staining, semi-high-throughput confocal imaging, and imaged-based quantification of 3D cultures. This protocol is versatile and can be applied in principle to any patient-derived material.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.