本研究旨在探讨不同规格吴茱萸（EF）甘草炮制对肠道黏膜微生物相关的肝脏炎症和氧化应激的影响。将25只昆明小鼠分为对照组（MCN）、生小花吴茱萸（ MRSEF）、生中花EF（MRMEF）、甘草加工小花EF（MLSEF）和甘草加工中花EF（MLSEF）组。 EF干预组给予不同规格的EF提取液灌胃。 21天后，测量小鼠肝脏炎症、氧化应激指标以及肠粘膜微生物群，并与MCN、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）进行比较MRMEF 中的水平显着增加。尽管MLSEF和MLMEF组氧化应激和炎症指标的变化趋势与原始EF组一致，但变化小于原始EF组。与原始 EF 组相比，MLSEF 和 MLMEF 显示出更接近 MCN 的代谢功能。 MRMEF中棒状杆菌的丰度显着低于MCN中的丰度，与甘草加工后的MCN没有显着差异。 MLSEF 中富含益生菌 Candidatus Arthromitus。 MLMEF 中富含益生菌乳酸菌。相关分析显示IL-1β、部分代谢功能与棒状杆菌之间存在显着负相关。中花EF对小鼠肝脏氧化应激和炎症因子的影响强于小花EF。甘草加工可以通过调节棒状杆菌的丰度和肠粘膜代谢功能来减少这种差异。版权所有 © 2024 梁、田、陈、张和彭。

This study aimed to investigate the effects of licorice processing of different Evodiae Fructus (EF) specifications on liver inflammation and oxidative stress associated with the intestinal mucosal microbiota.The 25 Kunming mice were divided into control (MCN), raw small-flowered Evodiae Fructus (MRSEF), raw medium-flowered EF (MRMEF), licorice-processed small-flowered EF (MLSEF), and licorice-processed medium-flowered EF (MLSEF) groups. The EF intervention groups were given different specifications of EF extract solutions by gavage. After 21 days, indices of liver inflammation and oxidative stress and intestinal mucosal microbiota were measured in mice.Compared with the MCN, malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels were significantly increased in the MRMEF. Although the trends of oxidative stress and inflammatory indexes in the MLSEF and MLMEF were consistent with those in the raw EF groups, the changes were smaller than those in the raw EF groups. Compared to the raw EF groups, the MLSEF and MLMEF showed closer approximations of metabolic function to the MCN. The abundance of Corynebacterium in MRMEF was significantly lower than that in the MCN, and it was not significantly different from the MCN after licorice processing. The probiotic Candidatus Arthromitus was enriched in the MLSEF. The probiotic Lactobacillus was enriched in the MLMEF. Correlation analysis revealed significant negative correlations between IL-1β, some metabolic functions and Corynebacterium.The effects of medium-flowered EF on oxidative stress and inflammatory factors in the liver of mice were stronger than those of small-flowered EF. The licorice processing can reduce this difference by modulating the abundance of Corynebacterium and intestinal mucosal metabolic function.Copyright © 2024 Liang, Tian, Chen, Zhang and Peng.