人类 T 细胞淋巴瘤 (TCL) 的组织病理学分类具有独特的突变基因分型，表明不同的淋巴瘤发生。假设在具有不同 TCL 表型的狗中也观察到类似的概念。本研究旨在鉴定先前报道的人类和狗在犬 TCL 和无效细胞淋巴瘤 (NCL) 中的单核苷酸多态性 (SNP)，并设计基于多重聚合酶链式反应从每个变体中提取出相容的寡核苷酸。从 68 个肿瘤样本（62 个 TCL 和 6 个 NCL）和 5 个 TCL 狗的血沉棕黄层样本中提取基因组 DNA。使用 MassARRAY 对 21 个基因的 44 个 SNP 中的四种 TCL 亚型和 NCL 进行了分析。在所有 TCL 亚型和 NCL 中观察到的 SNP 的最大发生率是 SATB1 c.1259A > C、KIT c.1275A > G、SEL1L c.2040  200C > G和TP53分别为c.1024C > T。一些 SNP 位置与 NCL 具有统计学显着相关性，包括 MYC p.S75F (p = 0.0003)、TP53 p.I149N (p = 0.030)、PDCD1 p.F37LX (p = 0.012) 和 POT1 p.R583* (p = 0.012)。每个 TCL 组织学亚型和 NCL 可能包含独特的突变基因谱，这可能在淋巴瘤基因风险因素中发挥作用，并可能有助于为每个犬类患者选择治疗靶点药物。版权所有 © 2024 Sirivisoot，Kasantikul， Techangamsuwan 和 Rungsipipat。

The histopathological classification of T-cell lymphoma (TCL) in humans has distinctive mutational genotyping that suggests different lymphomagenesis. A similar concept is assumed to be observed in dogs with different TCL phenotypes.This study aimed to identify the previously reported single-nucleotide polymorphisms (SNPs) in both human beings and dogs in canine TCLs and null-cell lymphomas (NCLs) and to design compatible oligonucleotides from each variant based on the multiplex polymerase chain reaction.Genomic DNA was extracted from 68 tumor specimens (62 TCLs and 6 NCLs) and 5 buffy coat samples from dogs with TCL. Four TCL subtypes and NCL were analyzed in 44 SNPs from 21 genes using the MassARRAY.The greatest incidences of SNPs observed in all TCL subtypes and NCL ware SATB1 c.1259A > C, KIT c.1275A > G, SEL1L c.2040 + 200C > G, and TP53 c.1024C > T, respectively. Some SNP locations were statistically significant associated with NCL, including MYC p.S75F (p = 0.0003), TP53 p.I149N (p = 0.030), PDCD1 p.F37LX (p = 0.012), and POT1 p.R583* (p = 0.012).Each TCL histological subtype and NCL are likely to contain distinctive mutational genetic profiles, which might play a role in lymphoma gene-risk factors and might be useful for selecting therapeutic target drugs for each canine patient.Copyright © 2024 Sirivisoot, Kasantikul, Techangamsuwan and Rungsipipat.