脓毒症引起的肾损伤构成重大威胁，需要有效的治疗策略。大麻二酚 (CBD) 通过表现出抗氧化和抗炎作用，对组织及其功能产生有益影响。本研究探讨了 CBD 在减轻 Wistar Albino 大鼠脂多糖 (LPS) 诱导的肾损伤中的潜在保护作用。将 32 只 Wistar Albino 大鼠分为对照组、LPS（腹腔注射 5 毫克/千克）、LPS  CBD 和 CBD（腹腔注射 5 毫克/千克）组。实验结束后，收集样本进行生化、遗传、组织病理学和免疫组织化学分析。氧化应激标志物如总氧化状态（TOS）和总抗氧化状态（TAS）、氧化应激指数（OSI）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、丙二醛（MDA）、免疫染色如肿瘤坏死因子α（ TNF-α)、白介素-10 (IL-10)、caspase-3、核因子红细胞 2 相关因子 2 (NRF2) 的基因表达、C/EBP 同源蛋白 (CHOP)、caspase-9、葡萄糖调节蛋白78 (GRP78)、B 细胞白血病/淋巴瘤 2 (Bcl2) 和组织组织学已进行了检查。 LPS 暴露组表现出明显的肾脏异常，LPS CBD 组的 CBD 干预减轻了这种异常。 CBD 降低了 TNF-α、caspase-3 和 IL-10 的免疫表达评分。从生化角度来看，CBD 诱导氧化平衡发生正向转变，增加 TAS、SOD 和 GPx，同时降低 TOS、OSI 和 MDA 水平。遗传分析强调了 CBD 对 NRF2、CHOP、caspase-9、GRP78 和 Bcl2 的调节影响，为了解其对 LPS 诱导的肾损伤的保护作用提供了分子见解。这项研究强调了 CBD 作为一种有前景的保护剂，可预防脓毒症引起的肾损伤。我们的研究结果可以为解决脓毒症肾脏并发症的潜在治疗途径提供有价值的见解。© 2024。作者获得 Springer-Verlag GmbH 德国（Springer Nature 旗下公司）的独家许可。

Sepsis-induced renal damage poses a significant threat, necessitating effective therapeutic strategies. Cannabidiol (CBD) has beneficial effects on tissues and their functions by exhibiting antioxidant and anti-inflammatory effects. This study investigates the potential protective effects of CBD in mitigating lipopolysaccharide (LPS)-induced renal injury in Wistar Albino rats. Thirty-two Wistar Albino rats were categorized into control, LPS (5 mg/kg i.p.), LPS + CBD, and CBD (5 mg/kg i.p.) groups. After the experiment, samples were collected for biochemical, genetic, histopathological, and immunohistochemical analyses. Oxidative stress markers as total oxidant status (TOS) and total antioxidant status (TAS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), immune staining as tumor necrosis factor alpha (TNF-α), interleukin-10 (IL-10), caspase-3, gene expressions as nuclear factor erythroid 2-related factor 2 (NRF2), C/EBP homologous protein (CHOP), caspase-9, glucose-regulating protein 78 (GRP78), B-cell leukemia/lymphoma 2 (Bcl2), and tissue histology have been examined. The LPS-exposed group exhibited significant renal abnormalities, mitigated by CBD intervention in the LPS + CBD group. CBD reduced immunoexpression scores for TNF-α, caspase-3, and IL-10. Biochemically, CBD induced a positive shift in the oxidative balance, increasing TAS, SOD, and GPx, while decreasing TOS, OSI, and MDA levels. Genetic analyses highlighted CBD's regulatory impact on NRF2, CHOP, caspase-9, GRP78, and Bcl2, providing molecular insights into its protective role against LPS-induced renal damage. This study underscores CBD as a promising protective agent against sepsis-induced renal damage. Our findings could provide valuable insights into potential therapeutic avenues for addressing renal complications in sepsis.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.