姜黄素（CUR）因其对人类健康的有益作用而受到全世界的关注。研究报告可能对多种癌症（包括胶质母细胞瘤）具有细胞毒性活性。到目前为止，人们很少关注 CUR 与脂质膜的结合特性，这会影响它们的电特性并可以深入了解它们的膜渗透能力。通过监测 zeta 电位和膜表面电荷与 pH 的关系，研究了多酚和体外模型（脂质体和 LN-18 人胶质母细胞瘤细胞）之间的生物物理相互作用。我们专注于实际测量，并对天然细胞膜中的相互作用进行理论分析。我们使用 MTT 测定来评估 CUR 处理细胞的活力。使用电泳光散射方法进行的测量证明了 CUR 对体外模型分析的膜表面电荷和 zeta 电位的剂量依赖性影响。我们根据溶液离子与胶质母细胞瘤细胞膜结合形成的吸附平衡的定量描述，确定了表征细胞膜的理论参数。 CUR 与脂质体和人类癌细胞的相互作用依赖于 pH 值。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Curcumin (CUR) has received worldwide attention for its beneficial effects on human health. Research report possible cytotoxic activity against various cancers, including glioblastoma. So far, little attention has been given to the binding properties of CUR to lipid membranes, which influences their electrical characteristics and can provide insight into their membrane-permeation abilities. Biophysical interactions between the polyphenol and in vitro models (liposomes and LN-18 human glioblastoma cells) were investigated by monitoring zeta potential and the membrane's surface charge as a function of pH. We focused on practical measurements and undertook a theoretical analysis of interactions in the natural cell membrane. We used the MTT assay to evaluate the viability of CUR-treated cells. Measurements performed using the Electrophoretic Light Scattering method demonstrated the dose-dependent effect of CUR on both membrane surface charge and zeta potential analyzed in vitro models. We determined theoretical parameters characterizing the cell membrane based on a quantitative description of the adsorption equilibria formed due to the binding of solution ions to the membrane of glioblastoma cells. The interaction of CUR with liposomes and human cancer cells is pH-dependent.Copyright © 2024 Elsevier B.V. All rights reserved.