类器官内的机械应力是疾病建模和药代动力学的关键指标，但目前的工具缺乏快速、动态筛选这些力学的能力。在这里，我们引入了生物相容性和可压缩的空心微型激光器，可实现类器官内细胞应力的全光学评估。激光光谱可以识别纳米尺度的细胞变形，相当于数十帕的应力敏感性。可压缩性使得能够研究各向同性分量，这是多细胞模型的基本力学。通过与微孔阵列集成，我们展示了类瘤中机械信号的高通量筛选，建立了机械响应药物筛选的平台。此外，我们展示了人类胚胎干细胞衍生的心脏类器官内动态收缩应力的监测和绘图，揭示了单个类器官内的内部机械不均匀性。这种方法消除了耗时的扫描和样品损坏，为类器官力学生物学提供了见解。

Mechanical stress within organoids is a pivotal indicator in disease modeling and pharmacokinetics, yet current tools lack the ability to rapidly and dynamically screen these mechanics. Here, we introduce biocompatible and compressible hollow microlasers that realize all-optical assessment of cellular stress within organoids. The laser spectroscopy yields identification of cellular deformation at the nanometer scale, corresponding to tens of pascals stress sensitivity. The compressibility enables the investigation of the isotropic component, which is the fundamental mechanics of multicellular models. By integrating with a microwell array, we demonstrate the high-throughput screening of mechanical cues in tumoroids, establishing a platform for mechano-responsive drug screening. Furthermore, we showcase the monitoring and mapping of dynamic contractile stress within human embryonic stem cell-derived cardiac organoids, revealing the internal mechanical inhomogeneity within a single organoid. This method eliminates time-consuming scanning and sample damage, providing insights into organoid mechanobiology.