乳腺癌 (MedBC) 是一种罕见的组织学类型，占所有浸润性乳腺癌的比例不到 5%。在这里，我们进行了一项探索性研究，旨在确定与非典型 MedBC 和 TNBC-TILS 肿瘤相比，成像质量流式细胞术 (IMC) 是否可用于表征罕见 MedBC 亚型的免疫浸润和空间分布异质性。在 MedBC 和 TNBC-TIL 中，肿瘤周围区域的免疫细胞显着富集，而在非典型 MedBC 中，免疫细胞表现出中心富集模式。浸润细胞的这种分布反映了与更有利的预后相关的主动免疫募集。在 MedBC 中，空间分析显示免疫细胞与肿瘤细胞的距离更远。 IMC 强调了乳腺肿瘤三种主要亚型的免疫微环境的异质性，有助于定义不同的免疫模式。© 2024。作者。

Medullary carcinoma of the breast (MedBC) is a rare histological type that accounts for less than 5% of all invasive breast cancers. Here, we performed an exploratory study aimed to determine whether imaging mass cytometry (IMC) can be used to characterize the immune infiltration and the spatial distribution heterogeneity in the rare subtype of MedBC compared to atypical MedBC and TNBC-TILS+ tumors. In both MedBC and TNBC-TILs+, there was a notable enrichment of immune cells in the peripheral regions of the tumors, whereas in atypical MedBC, the immune cells exhibited a central enrichment pattern. This distribution of infiltrated cells reflects an active immune recruitment correlated to more favorable prognosis. In MedBC, spatial analysis shows that immune cells are localized at a greater distance from the tumor cells. IMC highlights the heterogeneity of immune microenvironment across three main subtypes of breast tumors and could help to define distinct immune patterns.© 2024. The Author(s).