MicroRNA (miRNA) 是一种短的非编码核酸分子，在诊断和预测各种类型的癌症中发挥着重要作用。 miRNA 大量存在于皮肤间质液 (ISF) 中，提供实时和局部的生理信息。水凝胶微针 (HMN) 贴片能够以快速、无痛、微创且用户友好的方式收集 miRNA。在这项研究中，我们引入了一种基于荧光的 HMN 检测，即 HMN-miR 传感器，由甲基丙烯酸透明质酸 (MeHA) 和氧化石墨烯探针 DNA (GO.pDNA) 缀合物组成，用于 miR21 和 miR210 检测。 HMN-miR传感器表现出优异的皮肤穿透效率、快速的ISF收集能力以及足够的miRNA检测和序列识别特异性。 HMN-miR 传感器促进了一种新的检测，经过进一步优化，可以应用于未来的临床环境。其简单的制造工艺和优异的生物相容性使其在各种临床应用中具有巨大的潜力，例如个性化癌症治疗和监测烧伤创面的愈合进度。

MicroRNA (miRNA) is a type of short, non-coding nucleic acid molecule that plays essential roles in diagnosing and prognosing various types of cancer. MiRNA is abundantly present in skin interstitial fluid (ISF), providing real-time and localized physiological information. Hydrogel microneedle (HMN) patches enable miRNA collection in a fast, pain-free, minimally invasive, and user-friendly manner. In this study, we introduced a fluorescence-based HMN assay, namely the HMN-miR sensor, composed of methacrylated hyaluronic acid (MeHA) and a graphene oxide-probe DNA (GO.pDNA) conjugate for miR21 and miR210 detection. The HMN-miR sensor demonstrates excellent skin penetration efficiency, rapid ISF collection capability, and sufficient miRNA detection and sequence identification specificity. The HMN-miR sensor facilitates a new assay that, with further optimization, could be applied in future clinical settings. Its simple fabrication process and excellent biocompatibility give it significant potential for various clinical uses, such as personalized cancer treatment and monitoring the healing progress of burn wounds.