支气管内超声引导经支气管抽吸（EBUS-TBNA）已成为肺癌初始诊断和分期的标准。以前的指南一般侧重于 EBUS-TBNA 的“何时”和“如何”；然而，在采集期间和采集后处理和处理样本以帮助优化辅助研究的诊断率和组织完整性方面几乎没有可用的指导。本文件审查了有关 EBUS-TBNA 标本处理和处理的现有文献。采用严格的方法来提供值得信赖的循证指南和专家小组报告。小组成员利用 PICO（群体、干预、比较和结果）格式提出了关键的临床问题，解决了 EBUS-TBNA 标本处理中的特定主题。 MEDLINE（通过 PubMed）和 Cochrane 图书馆进行了系统检索，以识别相关文献，并辅以手动检索。使用文件评估工具对参考文献进行筛选，以评估纳入研究的质量，提取有意义的数据，并对支持每项推荐或建议的证据水平进行分级。我们对与以下相关的 9 个 PICO 问题的文献进行了系统回顾和批判性分析：处理和处理 EBUS-TBNA 标本得出了九项基于证据的陈述。处理和处理 EBUS-TBNA 标本的证据强度各不相同，但在某些方面是令人满意的，可以指导临床医生处理标本处理的某些方面。需要对样本处理和加工的许多方面进行更多研究，以帮助完善我们的知识库。版权所有 © 2024。由 Elsevier Inc. 出版。

Endobronchial Ultrasound-Guided Transbronchial Aspiration (EBUS-TBNA) has become the standard for initial lung cancer diagnosis and staging. Previous guidelines have generally focused on the "when" and "how" of EBUS-TBNA; however, little guidance is available on handling and processing specimens during and after acquisition to help optimize both diagnostic yield and tissue integrity for ancillary studies. This document examines the available literature on EBUS-TBNA specimen processing and handling.Rigorous methodology was applied to provide a trustworthy evidence-based guideline and expert panel report. Panelists developed key clinical questions utilizing the PICO (population, intervention, comparator, and outcome) format, addressing specific topics in EBUS-TBNA specimen processing. MEDLINE (via PubMed) and the Cochrane Library were systematically searched to identify relevant literature, supplemented by manual searches. References were screened for inclusion with document evaluation tools to assess the quality of included studies, extract meaningful data, and grade the level of evidence to support each recommendation or suggestion.Our systematic review and critical analysis of the literature of the 9 PICO questions related to handling and processing EBUS-TBNA specimens resulted in nine evidence-based statements.Evidence of the handling and processing of EBUS-TBNA specimens varies in strength but is satisfactory in some areas to guide clinicians in certain aspects of specimen handling. Additional research in many aspects of specimen handling and processing is needed to help improve our knowledge base.Copyright © 2024. Published by Elsevier Inc.