鳞状细胞癌 (SCC) 是一种常见的皮肤癌，通常由暴露于紫外线辐射 (UVR) 引起。最近的研究表明，DNA 甲基化的变化在癌症的发展中起着至关重要的作用。然而，SCC 的甲基化模式尚未得到很好的表征。识别发生鳞状细胞癌风险的生物标志物可能有助于早期检测和诊断，并有可能改善治疗和预防策略。本研究旨在调查暴露于 UVR 24 周的小鼠表皮甲基化变化。我们使用 Illumina Infinium 小鼠甲基化珠芯片检查了 260~199 个 CpG 的 DNA 甲基化水平，并在 24 周内每 4 周研究一次 UVR 暴露和未暴露小鼠的表皮（n = 39）。我们发现，暴露于 UVR 的小鼠和未暴露于 UVR 的小鼠之间的 CpG 甲基化水平（β 值）存在较大差异。我们还观察到这些小鼠的表观遗传年龄的差异。我们在 Rev、Ipmk、Rad51b、Fgfr2、Fgfr3 和 Ctnnb1 中鉴定出 CpG，它们可能作为 SCC 风险的潜在生物标志物，有助于早期发现和预防 SCC。需要进一步研究以确定这些 CpG 的生物学功能和临床意义。© 2024 作者。约翰·威利出版的《实验皮肤病学》

Squamous cell carcinoma (SCC) is a common skin cancer, often caused by exposure to ultraviolet radiation (UVR). Recent studies have shown that changes in DNA methylation play a crucial role in the development of cancers. However, methylation patterns of SCC are not well characterised. Identifying biomarkers for the risk of developing SCC could be helpful for early detection and diagnosis and can potentially improve treatment and prevention strategies. This study aimed to investigate methylation changes in the epidermis of mice exposed to UVR for 24 weeks. We examined the DNA methylation levels of 260 199 CpGs using the Illumina Infinium Mouse Methylation BeadChip and studied the epidermis of UVR-exposed and unexposed mice every 4 weeks for 24 weeks (n = 39). We identified CpGs with large differences in methylation levels (β-values) between UVR-exposed and unexposed mice. We also observed differences in the epigenetic age of these mice. We identified CpGs in Rev, Ipmk, Rad51b, Fgfr2, Fgfr3 and Ctnnb1 that may serve as potential biomarkers for SCC risk and could be helpful for the early detection and prevention of SCC. Further investigations are necessary to determine the biological functions and clinical significance of these CpGs.© 2024 The Author(s). Experimental Dermatology published by John Wiley & Sons Ltd.