食管鳞状细胞癌（ESCC）由于其预后不良且缺乏有效的治疗靶点，仍然是一个重大的临床挑战。环状 RNA (circRNA) 在癌症进展中至关重要。在这项研究中，采用高通量测序对食管鳞癌组织进行分析，发现hsa_circ_0001165在食管鳞癌肿瘤样本和细胞系中均显着升高，其表达与患者的TNM分期呈正相关。 hsa_circ_0001165 的敲低导致体外食管鳞癌细胞的恶性生物学行为减少，并且还抑制了体内肿瘤的生长。机制实验分析发现hsa_circ_0001165表达被真核翻译起始因子4A3（EIF4A3）正向增强。 Hsa_circ_0001165 作为 miR-381-3p 的 miRNA 海绵，通过一系列相关机制测定（包括双荧光素酶报告基因、RNA 免疫沉淀和 RNA 下拉）增加张力蛋白-3 (TNS3) 的表达。 ESCC组织中miR-381-3p表达下调，ESCC细胞增殖、侵袭和迁移受到抑制。 hsa_circ_0001165 表达上调可调节 miR-381-3p/TNS3 轴并促进 ESCC 的侵袭性表型。 Hsa_circ_0001165 被认为是 ESCC 的令人鼓舞的生物标志物和潜在治疗靶点，为诊断和治疗方法提供了创新选择。© 2024。作者。

Esophageal squamous cell carcinoma (ESCC) remains a major clinical challenge due to its poor prognosis and the scarcity effective therapeutic targets. Circular RNAs (circRNAs) are crucial in cancer progression. In this study, high-throughput sequencing was employed to profile ESCC tissues, revealing that hsa_circ_0001165 is notably elevated in both ESCC tumor samples and cell lines, with its expression is positively associated with patients' TNM staging. Knockdown of hsa_circ_0001165 resulted in reduced malignant biological behavior of ESCC cells in vitro and also inhibited tumor growth in vivo. Mechanism experimental analysis found that hsa_circ_0001165 expression is positively enhanced by eukaryotic translation initiation factor 4A3 (EIF4A3). Hsa_circ_0001165 acts as a miRNA sponge for miR-381-3p, increasing the expression of tensin-3 (TNS3) through a series of related mechanism assays include dual-luciferase reporter gene, RNA Immunoprecipitation and RNA-pulldown. The downregulation in miR-381-3p expression was observed in ESCC tissues, and the cell proliferation, invasion, and migration of ESCC were suppressed. The upregulated expression of hsa_circ_0001165 modulates the miR-381-3p/TNS3 axis and promotes aggressive phenotypes of ESCC. Hsa_circ_0001165 is regarded as a encouraging biomarker and potential therapeutic target for ESCC, presenting innovative options for both diagnostic and treatment approaches.© 2024. The Author(s).