男性前列腺衰老是一个不可避免的过程，前列腺会发生增生，这种增生可能会导致尿道受压，导致排尿功能障碍和相关症状，并增加患前列腺癌的风险。尽管前列腺衰老具有重要意义，但所涉及的分子机制仍不完全清楚。收集年轻（2 个月）和老年（24 个月）小鼠的前列腺裂片，用于单细胞 RNA 测序 (scRNA-seq) 分析。该研究包括前前列腺（AP）和腹侧/背侧/外侧前列腺（VDLP）的组织。数据分析包括使用统一流形近似和投影 (UMAP) 算法进行无监督聚类，以根据标记基因表达来识别不同的细胞类型。进行差异基因表达分析，以确定不同细胞类型的基因表达与年龄相关的变化。进行功能富集分析以阐明与差异表达基因相关的生物学途径。此外，还分析了细胞相互作用和发育轨迹，以表征前列腺衰老过程中的细胞动力学。衰老过程中小鼠前列腺的单细胞转录组分析揭示了各种细胞类型的异质性及其在衰老过程中的变化。我们发现老年小鼠的间充质细胞和免疫细胞的比例显着增加。我们的研究揭示了与细胞衰老、氧化应激和上皮细胞再生相关的基因和途径的改变。此外，我们观察到基底细胞可能经历上皮间质转化（EMT）成为间质细胞，在老年小鼠中尤其突出。此外，免疫细胞，特别是巨噬细胞和 T 细胞，在老年小鼠中表现出增强的炎症反应。 总之，我们的研究对老年和年轻小鼠前列腺的单细胞转录组进行了比较分析，阐明了老年小鼠和年轻小鼠前列腺之间的细胞和分子变化。老年和年轻小鼠前列腺。© 2024。作者。

Aging of the male prostate is an inevitable process in which the prostate undergoes hyperplasia, and this growth may lead to compression of the urethra, resulting in voiding dysfunction and associated symptoms, and an increased risk of prostate cancer. Despite the significance of prostate aging, the molecular mechanisms involved are still not fully understood.Prostate split by lobes from young (2 months) and aged (24 months) mice were collected for single-cell RNA sequencing (scRNA-seq) analysis. Tissues from both anterior prostate (AP) and ventral/dorsal/lateral prostate (VDLP) were included in the study. Data analysis included unsupervised clustering using the uniform manifold approximation and projection (UMAP) algorithm to identify distinct cell types based on marker gene expression. Differential gene expression analysis was performed to identify age-related changes in gene expression across different cell types. Functional enrichment analysis was conducted to elucidate biological pathways associated with differentially expressed genes. Additionally, cellular interactions and developmental trajectories were analyzed to characterize cellular dynamics during prostate aging.The single-cell transcriptome analysis of the mouse prostate during aging revealed heterogeneity across various cell types and their changes during the aging process. We found a significant increase in the proportion of mesenchymal and immune cells in aged mice. Our study unveiled alterations in genes and pathways associated with cellular senescence, oxidative stress, and regeneration in epithelial cells. Furthermore, we observed that basal cells may undergo epithelial-mesenchymal transition (EMT) to become mesenchymal cells, particularly prominent in aged mice. Additionally, immune cells, notably macrophages and T cells, exhibited a heightened inflammatory response in aged mice.In summary, our study provides a comparative analysis of the single-cell transcriptome of the aged and young mice prostates, elucidating cellular and molecular changes between the aged and young mice prostates.© 2024. The Author(s).