金纳米颗粒（GNP）的持续合成在生物医学应用中引起了广泛关注。在这项研究中，我们探索了补骨脂酚介导的金纳米粒子（Bak-GNP）的抗菌和抗癌潜力。补骨脂酚是补骨脂种子中发现的一种天然化合物，可作为 GNP 绿色合成的还原剂和稳定剂。我们的目标包括网络分析、分子对接、GNP 合成、Bak-GNP 对肺癌和肝癌的表征以及抗病原体和抗癌功效。分析蛋白质-蛋白质相互作用网络，以确定补骨脂酚在肺癌和肝癌中的有效蛋白质靶点。进行了分子对接研究，以验证目标蛋白对抗肺癌和肝癌的功效。此外，使用补骨脂酚合成了 Bak-GNP，并通过紫外可见光谱、动态光散射 (DLS)、zeta 电位透射电子显微镜 (TEM) 和傅里叶变换红外 (FTIR) 光谱等多种技术进行了表征，及其潜力GNAI3 成为最有希望的靶标，其结合能为 -7.5 kcal/mol，而 PTGER3 的结合能为 -6.9 kcal/mol，不同的表征技术揭示了 Bak-GNP 的成功合成。最低抑菌浓度 (MIC) 值证实，Bak-GNP 对革兰氏阳性菌和革兰氏阴性菌均表现出有效的抗菌活性。 Bak-GNPs 对 A549（肺癌）和 HepG2（肝癌）细胞具有显着的抗癌作用，IC50 值分别为 11.19 μg/mL 和 6.6 μg/mL。在两种细胞系中均观察到细胞凋亡的诱导和细胞增殖的抑制。活性氧 (ROS) 产生的增加有助于其抗癌作用。这项研究强调了补骨脂酚介导的 GNP 在生物医学方面的应用前景。这种使用补骨脂酚的绿色合成方法提供了一种可持续的方法来生产具有增强生物活性的纳米颗粒。需要进一步探索 Bak-GNP 的药理学特性和机制，以优化其临床使用的治疗功效。版权所有 © 2024 Mishra、Faruqui、Khanam、Khubaib、Ahmad、Saeed 和 Khan。

The sustained synthesis of gold nanoparticles (GNPs) has gained significant attention in biomedical applications. In this study, we explored the antibacterial and anticancer potential of bakuchiol-mediated gold nanoparticles (Bak-GNPs). Bakuchiol, a natural compound found in Psoralea corylifolia seeds, serves as both a reducing and stabilizing agent for green synthesis of GNPs. Our objectives include network analysis, molecular docking, synthesis of GNPs, characterization, and antipathogenic and anticancer efficacy of Bak-GNPs against lung and liver cancers.Protein-protein interaction networks were analyzed to identify effective protein targets for bakuchiol in lung and liver cancers. A molecular docking study was performed to validate the efficacy of the target protein against lung and liver cancer. Furthermore, Bak-GNPs were synthesized using bakuchiol and characterized by various techniques such as UV-visible spectroscopy, dynamic light scattering (DLS), zeta potential transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy, and their potential against pathogens and lung and liver cancers.GNAI3 emerged as the most promising target, with a binding energy of -7.5 kcal/mol compared to PTGER3's -6.9 kcal/mol, different characterization techniques revealed the successful synthesis of Bak-GNPs. Bak-GNPs exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria, as confirmed by minimum inhibitory concentration (MIC) values. Bak-GNPs demonstrated significant anticancer effects on A549 (lung cancer) and HepG2 (liver cancer) cells, with IC50 values of 11.19 μg/mL and 6.6 μg/mL, respectively. Induction of apoptosis and inhibition of cell proliferation were observed in both the cell lines. The increased production of reactive oxygen species (ROS) contributes to its anticancer effects.This study highlights promising biomedical applications of bakuchiol-mediated GNPs. This green synthesis approach using bakuchiol provides a sustainable method for producing nanoparticles with enhanced biological activities. Further exploration of the pharmacological properties and mechanisms of Bak-GNPs is required to optimize their therapeutic efficacy for clinical use.Copyright © 2024 Mishra, Faruqui, Khanam, Khubaib, Ahmad, Saeed and Khan.