转染效率是基因治疗和分子生物学中的一个关键参数，代表核酸导入靶细胞并表达的成功率。适体与基于纳米技术的递送系统的结合已证明治疗剂的转染效率显着提高，并且在推进基因治疗和开发包括癌症在内的各种疾病的靶向治疗方面具有巨大的潜力。在这项工作中，阳离子碳硅烷树突状系统被提出作为商业转染剂的替代品，证明当用于 apMNKQ2（一种针对癌症靶点选择的适体）的内化时，转染效率有所提高。它们的潜在治疗用途已在乳腺癌细胞系 MDA-MB-468 和 MDA-MB-231 中进行了评估，研究了纳米缀合物的细胞毒性、内化过程及其对细胞迁移过程的影响。© 2024 ）。高分子生物科学由 Wiley‐VCH GmbH 出版。

Transfection efficiency is a critical parameter in gene therapy and molecular biology, representing the success rate at which nucleic acids are introduced and expressed in target cells. The combination of aptamers with nanotechnology-based delivery systems has demonstrated remarkable improvements in the transfection efficiency of therapeutic agents and holds significant potential for advancing gene therapy and the development of targeted treatments for various diseases, including cancer. In this work, cationic carbosilane dendritic systems are presented as an alternative to commercial transfection agents, demonstrating an increase in transfection efficiency when used for the internalization of apMNKQ2, an aptamer selected against a target in cancer. Their potential therapeutic use has been evaluated in breast cancer cell lines, MDA-MB-468 and MDA-MB-231, studying the cytotoxicity of the nanoconjugate, the internalization process, and its effect on cellular migration processes.© 2024 The Author(s). Macromolecular Bioscience published by Wiley‐VCH GmbH.