人乳头瘤病毒 (HPV) 代表与多种癌症（特别是宫颈癌）相关的多种双链 DNA 病毒。高危类型的 HPV 通过将 DNA 整合到宿主基因组中而展现出致癌潜力。这种整合事件对基因组不稳定和恶性肿瘤的进展有显着贡献。然而，传统的检测方法，例如免疫组织化学或基于 PCR 的检测，面临着固有的挑战，因此正在开发替代工具来加快和简化分析。我们的研究引入了一种创新的生物传感平台，该平台将环介导扩增与电化学 (EC) 分析相结合，用于 HPV16 整合的特异性检测。通过靶向 E7 mRNA（HPV 整合中的核心角色）和整合时丢失的 E2 病毒基因转录物等关键元件，我们显示了 HPV16 的附加型和整合型之间的明显区别。我们的 EC 数据证实，在具有整合形式的病毒基因组的 HPV16 阳性细胞系中，E7 表达较高，而在具有完全整合的基因组的细胞中，E2 表达减少。此外，我们揭示了患者宫颈组织中不同的表达模式，与数字微滴 PCR、qRT-PCR 或免疫组织化学染色密切相关。因此，我们的平台提供了有关临床样本中 HPV 整合的见解，并促进宫颈癌研究和诊断的进一步进展。© 2024 作者。 《医学病毒学杂志》由 Wiley periodicals LLC 出版。

Human papillomaviruses (HPVs) represent a diverse group of double-stranded DNA viruses associated with various types of cancers, notably cervical cancer. High-risk types of HPVs exhibit their oncogenic potential through the integration of their DNA into the host genome. This integration event contributes significantly to genomic instability and the progression of malignancy. However, traditional detection methods, such as immunohistochemistry or PCR-based assays, face inherent challenges, and thus alternative tools are being developed to fasten and simplify the analysis. Our study introduces an innovative biosensing platform that combines loop-mediated amplification with electrochemical (EC) analysis for the specific detection of HPV16 integration. By targeting key elements like the E7 mRNA, a central player in HPV integration, and the E2 viral gene transcript lost upon integration, we show clear distinction between episomal and integrated forms of HPV16. Our EC data confirmed higher E7 expression in HPV16-positive cell lines having integrated forms of viral genome, while E2 expression was diminished in cells with fully integrated genomes. Moreover, we revealed distinct expression patterns in cervical tissue of patients, correlating well with digital droplet PCR, qRT-PCR, or immunohistochemical staining. Our platform thus offers insights into HPV integration in clinical samples and facilitates further advancements in cervical cancer research and diagnostics.© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.