R环是由RNA-DNA杂合体和置换的DNA链组成的三链核酸结构。它们分布广泛，在调节基因表达、DNA 复制以及 DNA 和组蛋白修饰中发挥着至关重要的作用。然而，它们的监管机制仍不清楚。随着 R 环检测技术的进步，在癌症模型中观察到 R 环水平的变化，通常与转录复制冲突和基因组不稳定有关。 N6-甲基腺苷 (m6A) 是一种 RNA 表观遗传修饰，通过影响 RNA 定位、剪接、翻译和降解来调节基因表达。通过查阅文献，我们发现带有 m6A 修饰的 R 环与肿瘤的发生和进展有关。本文综述了R-loops和m6A修饰在基因调控中的分子机制和检测方法，并综述了m6A修饰的R-loops在肿瘤学中的最新研究。我们的目标是为癌症基因组不稳定性的起源和靶向治疗的潜在策略提供新的见解。© 2024。作者。

R-loops are three-stranded nucleic acid structures composed of an RNA-DNA hybrid and a displaced DNA strand. They are widespread and play crucial roles in regulating gene expression, DNA replication, and DNA and histone modifications. However, their regulatory mechanisms remain unclear. As R-loop detection technology advances, changes in R-loop levels have been observed in cancer models, often associated with transcription-replication conflicts and genomic instability. N6-methyladenosine (m6A) is an RNA epigenetic modification that regulates gene expression by affecting RNA localization, splicing, translation, and degradation. Upon reviewing the literature, we found that R-loops with m6A modifications are implicated in tumor development and progression. This article summarizes the molecular mechanisms and detection methods of R-loops and m6A modifications in gene regulation, and reviews recent research on m6A-modified R-loops in oncology. Our goal is to provide new insights into the origins of genomic instability in cancer and potential strategies for targeted therapy.© 2024. The Author(s).