通过CRISPR/Cas9基因组编辑技术建立CIB1敲除人类多能干细胞系。
Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology.
发表日期：2024 Oct 15
作者：
Tingyu Gong, Dandan Liu, Xiaochen Wang, Danni Zhou, Ling Tang, Hao Wang, Jun Su, Ping Liang
来源：
Stem Cell Research & Therapy
摘要：
钙和整合素结合蛋白 1 (CIB1) 在许多不同的细胞类型和过程中发挥着多种作用，包括钙信号传导、迁移、粘附、增殖和存活。它与癌症、心血管疾病和男性不育症有关。本研究采用CRISPR/Cas9基因组编辑技术建立了CIB1敲除的人胚胎干细胞系，该细胞系表现出正常的多能性和核型。版权所有©2024 The Authors。由 Elsevier B.V. 出版。保留所有权利。
Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.