本研究重点是利用自由基将新单体 (E)-N-(4-(3-(4-溴苯基) 丙烯酰基) 苯基)-4-甲基苯磺酰胺 (Br-PS) 接枝到海藻酸钠 (Alg) 上聚合方法。研究了接枝聚合反应的最佳参数，包括引发剂和单体浓度、聚合反应持续时间和温度。此外，还计算了转化率、接枝率和固体含量百分比。使用傅里叶变换红外光谱 (FT-IR)、质子核磁共振 (1H NMR) 和扫描电子显微镜 (SEM) 对所得新型聚 (Br-PS)-g-Alg 进行了彻底分析。此外，使用以下方法测试了聚 (Br-PS)-g-Alg 对肺癌细胞系 (A549)、乳腺癌细胞系 (MDA-MB-231) 和正常细胞系 (MDCK) 的细胞毒性和选择性值中性红吸收试验。 Poly (Br-PS)-g-Alg 对 A549 和 MDA-MB-231 细胞系分别表现出有效的细胞毒性（IC50=33.37 和 40.9μg/mL）和高选择性（选择性指数=4.83 和 3.94）。此外，还制备了均匀的聚(Br-PS)-g-Alg珠，并研究了它们在各种介质中的溶胀率。这些珠子有可能作为 5-氟尿嘧啶 (5-FU) 的药物载体。模拟胃液 (SGF) 和肠液 (SIF) 中的释放实验显示，与 SIF 相比，SGF 中的 5-FU 释放模式较慢。使用具有 DFT/B3LYP/6-31(G) 基组的密度泛函理论从理论上验证了聚 (Br-PS)-g-Alg 的结构，揭示了由于不同官能团的存在而产生的不同相互作用。这项研究的结果可能会对新药物输送系统的开发产生重大影响。版权所有 © 2024。由 Elsevier B.V. 出版。

This study focuses on grafting a new monomer (E)-N-(4-(3-(4-bromophenyl) acryloyl) phenyl)-4-methyl benzene sulfonamide (Br-PS) onto sodium alginate (Alg) using a free radical polymerization method. The optimal parameters for the grafting polymerization reaction were investigated, including initiator and monomer concentrations, polymerization reaction duration, and temperature. Additionally, the conversion, graft, and solid content percentages were calculated. The resulting novel poly (Br-PS)-g-Alg was thoroughly analyzed using Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), and scanning electron microscopy (SEM). Moreover, poly (Br-PS)-g-Alg was tested for cytotoxicity and selectivity values on lung cancer cell lines (A549), breast cancer cell lines (MDA-MB-231), and a normal cell line (MDCK) using the neutral red uptake test. Poly (Br-PS)-g-Alg demonstrated potent cytotoxicity (IC50 = 33.37 and 40.9 μg/mL) and high selectivity (selectivity index = 4.83 and 3.94) on A549 and MDA-MB-231 cell lines, respectively. Furthermore, uniform beads of creative poly (Br-PS)-g-Alg were fabricated, and their swelling rate in various media was studied. These beads could potentially serve as drug carriers for 5-fluorouracil (5-FU). Release experiments in simulated gastric (SGF) and intestinal fluids (SIF) showed a slower 5-FU release pattern in SGF compared to SIF. The proposed structures of poly (Br-PS)-g-Alg were theoretically verified using density functional theory with DFT/B3LYP/6-31(G) basis set, revealing distinct interactions due to the presence of different functional groups. The findings of this study could significantly impact the development of new drug delivery systems.Copyright © 2024. Published by Elsevier B.V.