蝎毒可能含有具有潜在益处的药理物质。多项科学研究表明，特定的蝎毒可诱导细胞凋亡并抑制癌细胞的发展。本研究调查了来自Hottentotta saulcyi (H. saulcyi) 的粗毒液对体内小鼠模型和体外乳腺癌细胞的潜在抗癌特性。通过施加 8 和 10 V 电压的电刺激获得 H. saulcyi 蝎子的毒液。测定平均致死剂量 50 (LD50)。目前的工作使用荧光显微镜、MTT 测定和流式细胞术评估评估了 H. saulcyi 毒液的体外细胞毒性和形态特征。此外，还进行了研究以评估对乳腺癌小鼠模型的体内细胞毒性作用。在显微镜下对用蝎毒处理过的MCF-7细胞进行检查，发现存在正在凋亡的细胞。 H. saulcyi 的毒液具有抗癌特性，观察发现，当暴露于 1.47 mg/L 剂量时，MCF-7 细胞的凋亡率为 62.12%。基于所获得的结果，可以显示MCF-7细胞的存活率显着降低(P < 0.01)。此外，研究结果表明，H. saulcyi 的毒液导致 TNF-α、IL-6、IL-10、TGF-β 和 caspase 的合成显着增加（P<0.05）。与健康个体的对照组相比，施用 H. saulcyi 毒液的治疗组显示促凋亡基因的表达显着增强。与健康毒液治疗组（P<0.05）和恶性毒液治疗组（P<0.01）相比，健康对照组的BCL2基因转录表现出统计学上显着的增加。 H. saulcyi 的粗毒液在展示抗癌特性方面具有相当大的前景。可能需要进一步的研究来探索使用 H. saulcyi 粗毒液作为预防乳腺癌的药物平台的潜力。© 2024。作者。

Scorpion venom may include pharmacological substances that have the potential to provide benefits. Multiple scientific investigations have shown that particular scorpion venoms induce apoptosis and inhibit the development of cancerous cells. The present study investigated the potential anticancer properties of the crude venom derived from Hottentotta saulcyi (H. saulcyi) on both in vivo mice models and in vitro breast carcinoma cells. The venom of scorpions belonging to the species H. saulcyi was obtained with the application of electrical stimulation at voltages of 8 and 10 V. The determination of the Average Lethal Dose 50 (LD50) was conducted. The present work assessed the in vitro cytotoxicity and morphological characteristics of H. saulcyi venom using fluorescence microscopy, MTT assay, and flow cytometry assessment. Additionally, research was performed to assess the cytotoxic effects in vivo on a mouse model with breast cancer. The examination of MCF-7 cells treated with scorpion venom at a microscopic level revealed the existence of cells undergoing apoptosis. The venom of H. saulcyi has anticancer properties, as shown by the observation that MCF-7 cells had a 62.12% apoptotic rate when exposed to a dose of 1.47 mg/L. Based on the results obtained, it can be shown that the viability of MCF-7 cells has exhibited a substantial reduction (P < 0.01). Furthermore, the findings indicated that the venom of H. saulcyi resulted in a significant increase in the synthesis of TNF-α, IL-6, IL-10, TGF-β, and caspase (P < 0.05). The treatment groups administered with H. saulcyi venom exhibited a significant augmentation in the expression of proapoptotic genes compared to the control group of healthy individuals. The transcription of the BCL2 gene exhibited a statistically significant increase in the healthy control group compared to both the healthy venom-treated group (P < 0.05) and the malignant venom-treated group (P < 0.01). The crude venom of H. saulcyi has considerable promise in demonstrating anticancer properties. Further investigation may be warranted to explore the potential of using H. saulcyi crude venom as a medicinal platform for the prevention of breast cancer.© 2024. The Author(s).