体外分析表明，SARS-CoV-2 感染可差异调节结直肠癌细胞和前列腺癌细胞中的癌症样表型和细胞因子表达。
In vitro analysis suggests that SARS-CoV-2 infection differentially modulates cancer-like phenotypes and cytokine expression in colorectal and prostate cancer cells.
发表日期：2024 Oct 19
作者：
Alberta Serwaa, Fatima Oyawoye, Irene Amoakoh Owusu, Daniel Dosoo, Aaron Adom Manu, Augustine Kojo Sobo, Kwadwo Fosu, Charles Ochieng Olwal, Peter Kojo Quashie, Anastasia Rosebud Aikins
来源：
MOLECULAR & CELLULAR PROTEOMICS
摘要：
据报道，2019 年冠状病毒病 (COVID-19) 会加剧癌症结果。然而，人们对 COVID-19 如何影响癌症预后和发展仍知之甚少。在这里，我们研究了严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)（COVID-19 的病原体）对细胞癌症表型、癌症相关标记物和各种促炎细胞因子表达的影响。我们用刺突假病毒 (sPV) 和实验室储备的活 SARS-CoV-2 病毒感染表达血管紧张素转换酶 2 (ACE2) 的前列腺 (22RV1) 和结直肠 (DLD-1) 癌细胞系。感染后，我们量化了细胞癌症表型、一些癌症标志物（包括 Ki-67、BCL-2、VIM、MMP9 和 VEGF）以及促炎细胞因子的基因表达水平的变化。使用 MTT 和伤口愈合测定进行表型分析，而使用实时定量 PCR (RT-qPCR) 进行基因表达分析。我们发现，SARS-CoV-2 感染会影响前列腺癌细胞和结直肠癌细胞中涉及细胞生长、凋亡和迁移的几个关键细胞途径。我们的结果表明，SARS-CoV-2 感染确实会影响各种癌细胞表型以及分子癌症标志物和促炎细胞因子的表达，尽管是以细胞类型特异性的方式。我们的研究结果暗示需要进一步研究，并可能对评估其他病毒对癌症进展的影响产生影响。© 2024。作者。
The coronavirus disease 2019 (COVID-19) reportedly exacerbates cancer outcomes. However, how COVID-19 influences cancer prognosis and development remains poorly understood. Here, we investigated the effect of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), the etiological agent of COVID-19, on cellular cancer phenotypes the expression of cancer-related markers, and various proinflammatory cytokines. We infected prostate (22RV1) and colorectal (DLD-1) cancer cell lines, which express angiotensin-converting enzyme 2 (ACE2), with spike pseudovirus (sPV) and laboratory stocks of live SARS-CoV-2 viruses. After infection, we quantified changes in the cellular cancer phenotypes, the gene expression levels of some cancer markers, including Ki-67, BCL-2, VIM, MMP9, and VEGF, and proinflammatory cytokines. Phenotypic analysis was performed using MTT and wound healing assays, whereas gene expression analysis was carried out using real-time quantitative PCR (RT-qPCR). We show that SARS-CoV-2 infection impacts several key cellular pathways involved in cell growth, apoptosis, and migration, in prostate and colorectal cancer cells. Our results suggest that SARS-CoV-2 infection does influence various cancer cellular phenotypes and expression of molecular cancer markers and proinflammatory cytokines, albeit in a cell-type-specific manner. Our findings hint at the need for further studies and could have implications for evaluating the impact of other viruses on cancer progression.© 2024. The Author(s).