对乙酰氨基酚 (APAP) 引起的肝毒性是一种潜在危及生命的疾病。栀子果实提取物 (GJE) 含有京尼平苷 (Gen) 作为其主要活性成分，具有抗炎和抗氧化特性，可能有助于解决 APAP 诱导的肝毒性的潜在发病机制。本研究旨在评估GJE对APAP诱导的肝毒性小鼠模型的影响。24只雄性ICR小鼠分为4组（n = 6/组）： [1]对照组，小鼠给予蒸馏水； [1]对照组，小鼠给予蒸馏水； [2] APAP组，小鼠接受单剂量600 mg/kg APAP； [3] APAP  低剂量GJE组，小鼠接受APAP，30分钟后接受2剂低剂量GJE（0.44g/kg/剂，含Gen 100mg/kg/剂），间隔8小时； [4] APAP  高剂量GJE组，小鼠接受APAP，随后接受2剂高剂量GJE（0.88g/kg/剂，含Gen 200mg/kg/剂）。 APAP 给药 24 小时后，所有小鼠均被安乐死。肝组织用于组织学检查并测量谷胱甘肽（GSH）和丙二醛（MDA）水平。采用血清测定ALT和炎症细胞因子（肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6））水平。肝脏组织病理学显示APAP组出现中度至重度肝脏坏死性炎症，而APAP组仅出现轻度坏死性炎症。在两个治疗组中均观察到。与对照组相比，APAP 组的血清 ALT 水平显着升高，但在低剂量和高剂量 GJE 治疗后显着降低。 APAP组血清TNF-α水平显着高于对照组，并且在高剂量GJE治疗后显着降低（135.5±477.2 vs 35.5±25.8 vs 74.7±47.2 vs 41.4±50.8 pg/mL，分别）。血清 IL-6 也遵循类似的模式。与对照组相比，APAP 组的肝脏 GSH 水平显着降低，但在低剂量和高剂量 GJE 治疗后均显着升高（19.9±±4.5 vs. 81.5±±12.4 vs. 71.4±±7.8 vs. 82.6±±6.6 nmol/mg）分别为蛋白质）。相反，与对照组相比，APAP 组的肝脏 MDA 水平显着升高，但在高剂量 GJE 治疗后显着降低（分别为 108.6±201.5 vs. 40.5±18.0 vs. 40.5±16.8 nmol/mg 蛋白）。 G. jasminoides 果实提取物可能通过其抗炎和抗氧化特性来减轻 APAP 引起的肝毒性。© 2024。作者。

Acetaminophen (APAP)-induced hepatotoxicity is a potentially life-threatening condition. Gardenia jasminoides fruit extract (GJE), which contains geniposide (Gen) as its major active constituent, possesses anti-inflammatory and antioxidant properties and may help address the underlying pathogenesis of APAP-induced hepatotoxicity. This study aimed to evaluate the effects of GJE in a mouse model of APAP-induced hepatotoxicity.Twenty-four male ICR mice were divided into 4 groups (n = 6/group): [1] Control group, mice were given distilled water; [2] APAP group, mice received a single dose of 600 mg/kg APAP; [3] APAP + low-dose GJE group, mice received APAP followed 30 min later by 2 doses of low-dose GJE (0.44 g/kg/dose, containing Gen 100 mg/kg/dose) 8 h apart; [4] APAP + high-dose GJE group, mice received APAP followed by 2 doses of high-dose GJE (0.88 g/kg/dose, containing Gen 200 mg/kg/dose). All mice were euthanized 24 h after APAP administration. Liver tissue was used for histological examination and to measure glutathione (GSH) and malondialdehyde (MDA) levels. Serum was used to determine levels of ALT and inflammatory cytokines (tumor necrosis factor- α (TNF-α) and interleukin-6 (IL-6)).Liver histopathology showed moderate to severe hepatic necroinflammation in the APAP group, whereas only mild necroinflammation was observed in both treatment groups. Serum ALT levels were significantly elevated in the APAP group compared to the control group but were significantly reduced after low- and high-dose GJE treatment. Serum TNF- α levels were significantly higher in the APAP group than in the control group and were significantly lower after high-dose GJE treatment (135.5 ± 477.2 vs. 35.5 ± 25.8 vs. 74.7 ± 47.2 vs. 41.4 ± 50.8 pg/mL, respectively). Serum IL-6 followed a similar pattern. Hepatic GSH levels were significantly lower in the APAP group compared to the control group but significantly increased after both low- and high-dose GJE treatment (19.9 ± 4.5 vs. 81.5 ± 12.4 vs. 71.4 ± 7.8 vs. 82.6 ± 6.6 nmol/mg protein, respectively). Conversely, hepatic MDA levels were significantly elevated in the APAP group compared with the control group but significantly decreased after high-dose GJE treatment (108.6 ± 201.5 vs. 40.5 ± 18.0 vs. 40.5 ± 16.8 nmol/mg protein, respectively).Treatment with G. jasminoides fruit extract can alleviate APAP-induced hepatotoxicity, likely through its anti-inflammatory and antioxidant properties.© 2024. The Author(s).